Permeability of Biological Membranes by Gaspar Banfalvi

Author:Gaspar Banfalvi
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

(ii)Reliable membrane permeabilization was achieved by nano- and picosecond irradiation, allowing the penetration of fluorescent labeled antibodies into living cells. These results confirmed that the laser-induced permeabilization approach constituted a promising tool for targeted delivery of larger exogenous molecules into living cells (Yao et al. 2009).

(iii)The permeabilization of cell membranes and the introduction of fluorescein isothiocyanate-dextran and siRNA were enhanced using an optical field generated in the presence of polystyrene microspheres of 1000 nm diameter excited by a femtosecond laser pulse (Terakawa and Tanaka 2011). Gold nanoparticle mediated laser transfection served efficient siRNA mediated gene knock down (Heinemann et al. 2013). Optotransfection of mammalian cells based on femtosecond laser treatment was facilitated by gold nanorods (Ma et al. 2013).

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